MEng. (Oxford), PhD (Toronto), Post-Doc (Harvard, Stanford)
BioZone – Centre for Applied Bioscience and Bioengineering
Room: WB338 | Tel.: 416-978-7552 | Email: email@example.com
Tissue Engineering and Regenerative Medicine International Society
Cell behaviour in standard 2D plastic experimental systems is often not predictive of in vivo response. This is because cell behaviour is determined by a combination of intrinsic molecular properties and the local microenvironment, which in a tissue is complex and heterogeneous: tissues are composed of multiple cell types and matrix proteins organized into specific architectures. It is therefore not surprising that homogenous cell populations cultured in monolayers on the surface of plastic dishes, a microenvironment that has little resemblance to that in vivo, often fail to produce data predictive of cellular response in humans. Physiologically relevant, personalized, tissue mimetic systems offer the opportunity to systematically dissect fundamental mechanisms of tissue assembly and disease to allow identification of novel therapy targets to manipulate these processes for therapeutic benefit. Furthermore, such systems offer the potential to improve the effectiveness of therapy discovery and to enable the design of personalized treatments.
Our mission in the McGuigan lab is to use tissue-engineering strategies to assemble multicellular tissue mimetic platforms that are both physiologically relevant and allow acquisition of high value data for both drug discovery and fundamental research.
Our lab both develops technologies to control cell organization at the tissue scale and the cellular scale and strategies to stratify and visualize complex datasets from these heterogeneous tissue systems. Using these platforms, we are exploring the rules of tissue self-assembly, mechanisms of disease and the development of novel therapies.
An Engineered Patient-Derived Tumor Organoid Model That Can Be Disassembled to Study Cellular Responses in a Graded 3D Microenvironment, Natalie Landon-Brace, Jose L. Cadavid, Simon Latour, Ileana L. Co, Darren Rodenhizer, Nancy T. Li, Nila C. Wu, Eryn Bugbee, Aleks Chebotarev, Ji Zhang, Bradly G. Wouters, Alison P. McGuigan, Advanced Functional Materials 2021 (in press)
A three-dimensional engineered tumour for spatial snapshot analysis of cell metabolism and phenotype in hypoxic gradients., Rodenhizer D, Gaude E, Cojocari D, Mahadevan R, Frezza C, Wouters BG, McGuigan AP., Nat Mater. 2016 Feb;15(2):227-34. doi: 10.1038/nmat4482.
A TRACER 3D Co-Culture tumour model for head and neck cancer. Young M, Rodenhizer D, Dean T, D’Arcangelo E, Xu B, Ailles L, McGuigan AP. Biomaterials. 2018 May; 164: 54-69. doi: https://doi.org/10.1016/j.biomaterials.2018.01.038
Assembly of lung progenitors into developmentally-inspired geometry drives differentiation via cellular tension., Soleas JP, D’Arcangelo E, Huang L, Karoubi G, Nostro MC, McGuigan AP, Waddell TK., Biomaterials. 2020:120128.
Modulation of cellular polarization and migration by ephrin/Eph signal-mediated boundary formation., Javaherian S, D’Arcangelo E, Slater B, Londono C, Xu B, McGuigan AP., Integr Biol (Camb). 2017 Nov; 9(12): 934-946. doi: 10.1039/C7IB00176B.
Design of biomimetic substrates for long-term maintenance of alveolar epithelial cells. Poon JCH, Liao Z, Suzuki T, Carleton MM, Soleas JP, Aitchison JS, Karoubi G, Mcguigan AP, Waddell TK. Biomater Sci. 2018 Jan; 6(2):292-303. doi: 10.1039/C7BM00647K
Londono C, Lücker P, Loureiro JM, Soleas J, Slater B, Kabla A, McGuigan AP, Contact guidance behaviours of individual cells within confluent sheets cultured on nanogrooved substrates, Proc. Natl Acad Sci, USA 2014 ;111(5):1807-12