BioZone will be hosting Dr. Jens Kastenhofer, from our Dept. of Chemical Engineering & Applied Chemistry, on Thursday, October 28 from 3 pm – 4:30 pm.
Title: Extracellular production of recombinant proteins with E. coli
Escherichia coli is among the most favored expression hosts for recombinant proteins. It grows fast on cheap media, is easily genetically manipulated and bears low risk of contamination. However, the product is usually located inside the cell, resulting in a complex and costly purification process. Extracellular production may alleviate this bottleneck but is difficult to achieve due to the nature of the E. coli cell envelope and missing secretory pathways.
Dr. Jens Kastenhofer will present various strategies on how to achieve extracellular production of recombinant proteins with E. coli and demonstrate its benefit for the downstream process, both economical and ecological. Furthermore, the talk will explore approaches to monitoring extracellular production processes with E. coli.
Jens Kastenhofer obtained his MSc degree from Wageningen University & Research (The Netherlands) and his PhD from TU Wien (Vienna, Austria). His doctoral research was concerned with novel techniques for recombinant protein production in E. coli. He was awarded with the Erwin-Schrödinger-Fellowship from the Austrian Science Fund (FWF) for a postdoc position at the Department of Chemical Engineering & Applied Chemistry, University of Toronto. In the lab of Prof. D. Grant Allen, he is working towards understanding the response of microalgae to rare earth elements.